Sterility:Article Title: Replacing fishmeal protein with blended alternatives alters growth, feed utilization, protein deposition and gut micromorphology of humpback grouper, Cromileptes altivelis
Article Snippet: This research aims to investigate the effects of different dietary fishmeal (FM) protein replacements by blended alternative proteins on growth, feed utilization, protein deposition and gut micromorphology of humpback grouper, Cromileptes altivelis.. Five isoenergetic (14.23 kJ/g, dry matter), isoproteic (48.91 %, dry matter) and isolipidic (10 %, dry matter) experimental diets were formulated, with 0 % (R0), 21 % (R21), 42 % (R42), 63 % (R63) and 84 % (R84) FM protein being replaced by blended alternative proteins which consisted of soybean protein isolate (SPI), poultry by-product meal (PBM), hemoglobin powder (HP).. Triplicate groups of fish (mean initial body weight: 6.50 ± 0.17 g, 12 fish/group) were manually fed each diet to apparent satiety twice daily for 8 weeks.
Article Title: Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes.
Article Snippet: .. AGENT or RESOURCE SOURCE IDENTIFIER emicals, peptides, and recombinant proteins thylthioninium chloride injection Jumpcan Pharmaceutical Cat. H32024827 ecoxib sodium for injection Qilu Pharmaceutical Cat.H20183180 D Isoflurane solution RWD Life Science Cat.R510-22-10 alube vet ophthalmic ointment Dechra Cat.17033-211-38 e hair removal cream for sensitive skin Veet N/A Paraformaldehyde fixative solution Servicebio Cat.G1101-500ML siological saline solution (0.9%, sterile) Biosharp Cat.BL158A matoxylin staining solution Servicebio Cat.CR2112069 in staining solution Servicebio Cat.CR2202010 idone iodine disinfectant Shanghai Likang High-Tech Cat.8100062 erimental models: Organisms/strains use, C57BL/6J, 6 weeks, male SPF Biotech N/A er hthalmic forceps (straight and curved) RWD Life Science Cat.F13014-12 (Continued on next page) STAR Protocols 5, 103487, December 20, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dissecting scissors RWD Life Science Cat.S12029-11 Dedicate forceps RWD Life Science Cat.F11027-13 Retractor RWD Life Science Cat.R22029-03 R500 Small animal anesthesia machine RWD Life Science N/A Gas filter Canister RWD Life Science N/A Air pump RWD Life Science N/A R415 VentStar small animal ventilator RWD Life Science N/A ThermoStar Heating pad RWD Life Science N/A 22G endotracheal tube RWD Life Science N/A 4–0 Absorbable surgical sutures JinHuan Medical N/A Sterile single-use syringes for insulin (30G) Shanghai KDL Group N/A ll OPEN ACCESS Protocol ..
Staining:Article Title: The Immunomodulatory Effects of Porcupine Bezoar on Cyclophosphamide-Induced Immunosuppression in Rats
Article Snippet: The water used in the experiments was sourced from Hangzhou Wahaha Group Co., Ltd. (Hangzhou, China). .. The following reagents were obtained from Servicebio Technology Co., Ltd. (Wuhan, China): Paraformaldehyde Fixative Solution, Hematoxylin Staining Solution, Hematoxylin Differentiation Solution, Hematoxylin Bluing Solution, and Eosin Staining Solution. .. Enzyme-Linked Immunosorbent Assay (ELISA) kits were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (Shanghai, China).
Article Title: Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes.
Article Snippet: .. AGENT or RESOURCE SOURCE IDENTIFIER emicals, peptides, and recombinant proteins thylthioninium chloride injection Jumpcan Pharmaceutical Cat. H32024827 ecoxib sodium for injection Qilu Pharmaceutical Cat.H20183180 D Isoflurane solution RWD Life Science Cat.R510-22-10 alube vet ophthalmic ointment Dechra Cat.17033-211-38 e hair removal cream for sensitive skin Veet N/A Paraformaldehyde fixative solution Servicebio Cat.G1101-500ML siological saline solution (0.9%, sterile) Biosharp Cat.BL158A matoxylin staining solution Servicebio Cat.CR2112069 in staining solution Servicebio Cat.CR2202010 idone iodine disinfectant Shanghai Likang High-Tech Cat.8100062 erimental models: Organisms/strains use, C57BL/6J, 6 weeks, male SPF Biotech N/A er hthalmic forceps (straight and curved) RWD Life Science Cat.F13014-12 (Continued on next page) STAR Protocols 5, 103487, December 20, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dissecting scissors RWD Life Science Cat.S12029-11 Dedicate forceps RWD Life Science Cat.F11027-13 Retractor RWD Life Science Cat.R22029-03 R500 Small animal anesthesia machine RWD Life Science N/A Gas filter Canister RWD Life Science N/A Air pump RWD Life Science N/A R415 VentStar small animal ventilator RWD Life Science N/A ThermoStar Heating pad RWD Life Science N/A 22G endotracheal tube RWD Life Science N/A 4–0 Absorbable surgical sutures JinHuan Medical N/A Sterile single-use syringes for insulin (30G) Shanghai KDL Group N/A ll OPEN ACCESS Protocol ..
Article Title: The Immunomodulatory Effects of Porcupine Bezoar on Cyclophosphamide-Induced Immunosuppression in Rats
Article Snippet: The water used in the experiments was sourced from Hangzhou Wahaha Group Co., Ltd. (Hangzhou, China). .. The following reagents were obtained from Servicebio Technology Co., Ltd. (Wuhan, China): Paraformaldehyde Fixative Solution, Hematoxylin Staining Solution, Hematoxylin Differentiation Solution, Hematoxylin Bluing Solution, and Eosin Staining Solution. .. Enzyme-Linked Immunosorbent Assay (ELISA) kits were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (Shanghai, China).
Recombinant:Article Title: Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes.
Article Snippet: .. AGENT or RESOURCE SOURCE IDENTIFIER emicals, peptides, and recombinant proteins thylthioninium chloride injection Jumpcan Pharmaceutical Cat. H32024827 ecoxib sodium for injection Qilu Pharmaceutical Cat.H20183180 D Isoflurane solution RWD Life Science Cat.R510-22-10 alube vet ophthalmic ointment Dechra Cat.17033-211-38 e hair removal cream for sensitive skin Veet N/A Paraformaldehyde fixative solution Servicebio Cat.G1101-500ML siological saline solution (0.9%, sterile) Biosharp Cat.BL158A matoxylin staining solution Servicebio Cat.CR2112069 in staining solution Servicebio Cat.CR2202010 idone iodine disinfectant Shanghai Likang High-Tech Cat.8100062 erimental models: Organisms/strains use, C57BL/6J, 6 weeks, male SPF Biotech N/A er hthalmic forceps (straight and curved) RWD Life Science Cat.F13014-12 (Continued on next page) STAR Protocols 5, 103487, December 20, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dissecting scissors RWD Life Science Cat.S12029-11 Dedicate forceps RWD Life Science Cat.F11027-13 Retractor RWD Life Science Cat.R22029-03 R500 Small animal anesthesia machine RWD Life Science N/A Gas filter Canister RWD Life Science N/A Air pump RWD Life Science N/A R415 VentStar small animal ventilator RWD Life Science N/A ThermoStar Heating pad RWD Life Science N/A 22G endotracheal tube RWD Life Science N/A 4–0 Absorbable surgical sutures JinHuan Medical N/A Sterile single-use syringes for insulin (30G) Shanghai KDL Group N/A ll OPEN ACCESS Protocol ..
Injection:Article Title: Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes.
Article Snippet: .. AGENT or RESOURCE SOURCE IDENTIFIER emicals, peptides, and recombinant proteins thylthioninium chloride injection Jumpcan Pharmaceutical Cat. H32024827 ecoxib sodium for injection Qilu Pharmaceutical Cat.H20183180 D Isoflurane solution RWD Life Science Cat.R510-22-10 alube vet ophthalmic ointment Dechra Cat.17033-211-38 e hair removal cream for sensitive skin Veet N/A Paraformaldehyde fixative solution Servicebio Cat.G1101-500ML siological saline solution (0.9%, sterile) Biosharp Cat.BL158A matoxylin staining solution Servicebio Cat.CR2112069 in staining solution Servicebio Cat.CR2202010 idone iodine disinfectant Shanghai Likang High-Tech Cat.8100062 erimental models: Organisms/strains use, C57BL/6J, 6 weeks, male SPF Biotech N/A er hthalmic forceps (straight and curved) RWD Life Science Cat.F13014-12 (Continued on next page) STAR Protocols 5, 103487, December 20, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dissecting scissors RWD Life Science Cat.S12029-11 Dedicate forceps RWD Life Science Cat.F11027-13 Retractor RWD Life Science Cat.R22029-03 R500 Small animal anesthesia machine RWD Life Science N/A Gas filter Canister RWD Life Science N/A Air pump RWD Life Science N/A R415 VentStar small animal ventilator RWD Life Science N/A ThermoStar Heating pad RWD Life Science N/A 22G endotracheal tube RWD Life Science N/A 4–0 Absorbable surgical sutures JinHuan Medical N/A Sterile single-use syringes for insulin (30G) Shanghai KDL Group N/A ll OPEN ACCESS Protocol ..
Ointment:Article Title: Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes.
Article Snippet: .. AGENT or RESOURCE SOURCE IDENTIFIER emicals, peptides, and recombinant proteins thylthioninium chloride injection Jumpcan Pharmaceutical Cat. H32024827 ecoxib sodium for injection Qilu Pharmaceutical Cat.H20183180 D Isoflurane solution RWD Life Science Cat.R510-22-10 alube vet ophthalmic ointment Dechra Cat.17033-211-38 e hair removal cream for sensitive skin Veet N/A Paraformaldehyde fixative solution Servicebio Cat.G1101-500ML siological saline solution (0.9%, sterile) Biosharp Cat.BL158A matoxylin staining solution Servicebio Cat.CR2112069 in staining solution Servicebio Cat.CR2202010 idone iodine disinfectant Shanghai Likang High-Tech Cat.8100062 erimental models: Organisms/strains use, C57BL/6J, 6 weeks, male SPF Biotech N/A er hthalmic forceps (straight and curved) RWD Life Science Cat.F13014-12 (Continued on next page) STAR Protocols 5, 103487, December 20, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dissecting scissors RWD Life Science Cat.S12029-11 Dedicate forceps RWD Life Science Cat.F11027-13 Retractor RWD Life Science Cat.R22029-03 R500 Small animal anesthesia machine RWD Life Science N/A Gas filter Canister RWD Life Science N/A Air pump RWD Life Science N/A R415 VentStar small animal ventilator RWD Life Science N/A ThermoStar Heating pad RWD Life Science N/A 22G endotracheal tube RWD Life Science N/A 4–0 Absorbable surgical sutures JinHuan Medical N/A Sterile single-use syringes for insulin (30G) Shanghai KDL Group N/A ll OPEN ACCESS Protocol ..
Cream:Article Title: Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes.
Article Snippet: .. AGENT or RESOURCE SOURCE IDENTIFIER emicals, peptides, and recombinant proteins thylthioninium chloride injection Jumpcan Pharmaceutical Cat. H32024827 ecoxib sodium for injection Qilu Pharmaceutical Cat.H20183180 D Isoflurane solution RWD Life Science Cat.R510-22-10 alube vet ophthalmic ointment Dechra Cat.17033-211-38 e hair removal cream for sensitive skin Veet N/A Paraformaldehyde fixative solution Servicebio Cat.G1101-500ML siological saline solution (0.9%, sterile) Biosharp Cat.BL158A matoxylin staining solution Servicebio Cat.CR2112069 in staining solution Servicebio Cat.CR2202010 idone iodine disinfectant Shanghai Likang High-Tech Cat.8100062 erimental models: Organisms/strains use, C57BL/6J, 6 weeks, male SPF Biotech N/A er hthalmic forceps (straight and curved) RWD Life Science Cat.F13014-12 (Continued on next page) STAR Protocols 5, 103487, December 20, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dissecting scissors RWD Life Science Cat.S12029-11 Dedicate forceps RWD Life Science Cat.F11027-13 Retractor RWD Life Science Cat.R22029-03 R500 Small animal anesthesia machine RWD Life Science N/A Gas filter Canister RWD Life Science N/A Air pump RWD Life Science N/A R415 VentStar small animal ventilator RWD Life Science N/A ThermoStar Heating pad RWD Life Science N/A 22G endotracheal tube RWD Life Science N/A 4–0 Absorbable surgical sutures JinHuan Medical N/A Sterile single-use syringes for insulin (30G) Shanghai KDL Group N/A ll OPEN ACCESS Protocol ..
Saline:Article Title: Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes.
Article Snippet: .. AGENT or RESOURCE SOURCE IDENTIFIER emicals, peptides, and recombinant proteins thylthioninium chloride injection Jumpcan Pharmaceutical Cat. H32024827 ecoxib sodium for injection Qilu Pharmaceutical Cat.H20183180 D Isoflurane solution RWD Life Science Cat.R510-22-10 alube vet ophthalmic ointment Dechra Cat.17033-211-38 e hair removal cream for sensitive skin Veet N/A Paraformaldehyde fixative solution Servicebio Cat.G1101-500ML siological saline solution (0.9%, sterile) Biosharp Cat.BL158A matoxylin staining solution Servicebio Cat.CR2112069 in staining solution Servicebio Cat.CR2202010 idone iodine disinfectant Shanghai Likang High-Tech Cat.8100062 erimental models: Organisms/strains use, C57BL/6J, 6 weeks, male SPF Biotech N/A er hthalmic forceps (straight and curved) RWD Life Science Cat.F13014-12 (Continued on next page) STAR Protocols 5, 103487, December 20, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dissecting scissors RWD Life Science Cat.S12029-11 Dedicate forceps RWD Life Science Cat.F11027-13 Retractor RWD Life Science Cat.R22029-03 R500 Small animal anesthesia machine RWD Life Science N/A Gas filter Canister RWD Life Science N/A Air pump RWD Life Science N/A R415 VentStar small animal ventilator RWD Life Science N/A ThermoStar Heating pad RWD Life Science N/A 22G endotracheal tube RWD Life Science N/A 4–0 Absorbable surgical sutures JinHuan Medical N/A Sterile single-use syringes for insulin (30G) Shanghai KDL Group N/A ll OPEN ACCESS Protocol ..
TUNEL Assay:Article Title: PTEN restoration and CXCR2 depletion synergistically enhance the effect of enzalutamide and inhibit bone metastatic CRPC
Article Snippet: Enzalutamide (Enz) was generously provided by Shanghai Fosun Pharmaceutical Group Co., Ltd. PTEN plasmid, EGFP plasmid, Cy3-siRNA, siFAM and CXCR2 siRNA were purchased from GenePharma (Shanghai, China). .. The 4% paraformaldehyde fixative solution (Servicebio), TUNEL apoptosis assay kit, Ki67 cell proliferation assay kit and Alexa Fluor 488-labeled goat anti-rabbit IgG (H + L) secondary antibody were obtained from Servicebio. .. A Cell Counting Kit-8 (CCK-8), propidium iodide (PI), 4',6-diamidino-2-phenylindole (DAPI), 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO, λex/λem = 484/501 nm), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD, λex/λem = 644/655 nm), LysoTracker Red (λex/λem = 577/590 nm) and an Annexin V-FITC apoptosis detection kit were purchased from Beyotime Biotechnology.
Article Title: PTEN restoration and CXCR2 depletion synergistically enhance the effect of enzalutamide and inhibit bone metastatic CRPC
Article Snippet: Enzalutamide (Enz) was generously provided by Shanghai Fosun Pharmaceutical Group Co., Ltd. PTEN plasmid, EGFP plasmid, Cy3-siRNA, siFAM and CXCR2 siRNA were purchased from GenePharma (Shanghai, China). .. The 4% paraformaldehyde fixative solution (Servicebio), TUNEL apoptosis assay kit, Ki67 cell proliferation assay kit and Alexa Fluor 488-labeled goat anti-rabbit IgG (H + L) secondary antibody were obtained from Servicebio. ..
Apoptosis Assay:Article Title: PTEN restoration and CXCR2 depletion synergistically enhance the effect of enzalutamide and inhibit bone metastatic CRPC
Article Snippet: Enzalutamide (Enz) was generously provided by Shanghai Fosun Pharmaceutical Group Co., Ltd. PTEN plasmid, EGFP plasmid, Cy3-siRNA, siFAM and CXCR2 siRNA were purchased from GenePharma (Shanghai, China). .. The 4% paraformaldehyde fixative solution (Servicebio), TUNEL apoptosis assay kit, Ki67 cell proliferation assay kit and Alexa Fluor 488-labeled goat anti-rabbit IgG (H + L) secondary antibody were obtained from Servicebio. .. A Cell Counting Kit-8 (CCK-8), propidium iodide (PI), 4',6-diamidino-2-phenylindole (DAPI), 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO, λex/λem = 484/501 nm), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD, λex/λem = 644/655 nm), LysoTracker Red (λex/λem = 577/590 nm) and an Annexin V-FITC apoptosis detection kit were purchased from Beyotime Biotechnology.
Article Title: PTEN restoration and CXCR2 depletion synergistically enhance the effect of enzalutamide and inhibit bone metastatic CRPC
Article Snippet: Enzalutamide (Enz) was generously provided by Shanghai Fosun Pharmaceutical Group Co., Ltd. PTEN plasmid, EGFP plasmid, Cy3-siRNA, siFAM and CXCR2 siRNA were purchased from GenePharma (Shanghai, China). .. The 4% paraformaldehyde fixative solution (Servicebio), TUNEL apoptosis assay kit, Ki67 cell proliferation assay kit and Alexa Fluor 488-labeled goat anti-rabbit IgG (H + L) secondary antibody were obtained from Servicebio. ..
Proliferation Assay:Article Title: PTEN restoration and CXCR2 depletion synergistically enhance the effect of enzalutamide and inhibit bone metastatic CRPC
Article Snippet: Enzalutamide (Enz) was generously provided by Shanghai Fosun Pharmaceutical Group Co., Ltd. PTEN plasmid, EGFP plasmid, Cy3-siRNA, siFAM and CXCR2 siRNA were purchased from GenePharma (Shanghai, China). .. The 4% paraformaldehyde fixative solution (Servicebio), TUNEL apoptosis assay kit, Ki67 cell proliferation assay kit and Alexa Fluor 488-labeled goat anti-rabbit IgG (H + L) secondary antibody were obtained from Servicebio. .. A Cell Counting Kit-8 (CCK-8), propidium iodide (PI), 4',6-diamidino-2-phenylindole (DAPI), 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO, λex/λem = 484/501 nm), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD, λex/λem = 644/655 nm), LysoTracker Red (λex/λem = 577/590 nm) and an Annexin V-FITC apoptosis detection kit were purchased from Beyotime Biotechnology.
Article Title: PTEN restoration and CXCR2 depletion synergistically enhance the effect of enzalutamide and inhibit bone metastatic CRPC
Article Snippet: Enzalutamide (Enz) was generously provided by Shanghai Fosun Pharmaceutical Group Co., Ltd. PTEN plasmid, EGFP plasmid, Cy3-siRNA, siFAM and CXCR2 siRNA were purchased from GenePharma (Shanghai, China). .. The 4% paraformaldehyde fixative solution (Servicebio), TUNEL apoptosis assay kit, Ki67 cell proliferation assay kit and Alexa Fluor 488-labeled goat anti-rabbit IgG (H + L) secondary antibody were obtained from Servicebio. ..
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